Materials and methods for the alteration of enzyme and acetyl CoA levels in plants

Materials and methods for the alteration of enzyme and acetyl CoA levels in plants" (2009). Iowa State University Patents. Paper 30. Nikolau et al. (*) Notice: Subject to any disclaimer, the term of this patent is extended or adjusted under 35 U.S.C. 154(b) by 393 days. wo wo wo wo wo wo wo wo wo See application file for complete search history. ABSTRACT The present invention provides nucleic acid and amino acid sequences of acetyl CoA synthetase (ACS), plastidic pyru-vate dehydrogenase (pPDH), ATP citrate lyase (ACL), Ara-bidopsis pyruvate decarboxylase (PDC), and Arabidopsis aldehyde dehydrogenase (ALDH), specifically ALDH-2 and ALDH-4. The present invention also provides a recombinant vector comprising a nucleic acid sequence encoding one of the aforementioned enzymes, an antisense sequence thereto or a ribozyme therefor, a cell transformed with such a vector, antibodies to the enzymes, a plant cell, a plant tissue, a plant organ or a plant in which the level of an enzyme has been altered, and a method of producing such a plant cell, plant tissue, plant organ or plant. Desirably, alterationofthe level of enzyme results in an alteration of the level of acetyl CoA in the plant cell, plant tissue, plant organ or plant. In addition, the present invention provides a recombinant vector comprising an antisense sequence of a nucleic acid sequence encoding pyruvate decarboxylase (PDC), theE 1 a subunit of pPD H, the El ~subunit ofpPDH, the E2 subunit of pPDH, mitochon-drial pyruvate dehydrogenase (mtPDH) or aldehyde dehydro-genase (ALDH) or a ribozyme that can cleave an RNA molecule encoding PDC, Ela pPDH, El~ pPDH, E2 pPDH, mtPDH or ALDH. 2341 caaattaatt aaaagttaca aaaatataaa aaaaaaaaaa a a [SEQ ID NO: 1]